实验动物科学 ›› 2023, Vol. 40 ›› Issue (6): 47-52.DOI: 10. 3969 / j. issn. 1006-6179. 2023. 06. 009

• 论著 • 上一篇    下一篇

比较 5 种消毒剂对 4 种细菌的杀菌效果

  

  1. ( 1. 中国食品药品检定研究院,北京 102629) ( 2. 中国兽医药品监察所,北京 100081)
  • 收稿日期:2022-04-01 出版日期:2023-12-28 发布日期:2024-01-08
  • 通讯作者: 马丽颖( 1972—) ,女,主任技师,研究方向:实验动物设施运行管理. E-mail:maliyinglw@ hotmail. com
  • 作者简介:刘志国( 1989—) ,男,执业兽医师,研究方向:实验动物兽医与饲养管理. E-mail:804819660@ qq. com

Comparison of Bactericidal Effects of 5 Disinfectants on 4 Kinds of Bacteria

  1. ( 1. National Institutes for Food and Drug Control, Beijing 102629, China)
    ( 2. China Institute of Veterinary Drug Control, Beijing 100081, China)
  • Received:2022-04-01 Online:2023-12-28 Published:2024-01-08

摘要: 目的 验证 5 种消毒剂对 4 种 SPF 级实验动物必须排除的细菌的杀菌效果。 方法 以 D / E 中和肉汤为中和剂,检测其对 5 种消毒剂的中和作用;通过悬液定量杀菌实验,测试 5 种消毒剂对金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌和鼠伤寒沙门氏菌的杀菌效果。 结果 D / E 中和肉汤对于新洁尔灭、健之素消毒液、百毒杀、百胜-30 和 75%乙醇均有较好的中和作用。 新洁尔灭、健之素消毒液、百胜-30 和 75%乙醇 4 种消毒剂分别与 4 种细菌作用,1. 5 min 后杀灭效果良好。 1 ∶ 600 倍稀释的百毒杀消毒液有效杀灭金黄色葡萄球菌、肺炎克雷伯菌、沙门氏菌需要 5 ~ 10 min,但它对铜绿假单胞菌杀菌效果不理想。 1 ∶ 200 倍稀释的百毒杀消毒液作用 1. 5 min,可以有效杀灭铜绿假单胞菌。 结论 新洁尔灭、健之素消毒液、百胜-30 和 75%乙醇在较短时间内即可对 4 种细菌产生良好的消毒效果。 百毒杀有效杀灭铜绿假单胞菌需要较高浓度。

关键词: 消毒剂, 金黄色葡萄球菌, 铜绿假单胞菌, 肺炎克雷伯菌, 沙门氏菌, 杀菌效果

Abstract: Objective To verify the bactericidal effect of 5 kinds of disinfectants on 4 kinds of bacteria which must be excluded in SPF experiment animals, this study was performed. Method D / E neutralizing broth was used as neutralizing agent to investigated its neutralizing effect on 5 disinfectants. The bactericidal effects of the 5 disinfectants on Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhimurium were investigated by suspension quantitative bactericidal test. Result D / E neutralization broth had good neutralization effect on bromogeramine solution, Jianzhisu disinfectant, Didecyl dimethyl ammonium bromide solution, BIOCID-30 and 75% alcohol solution. The bromogeramine solution, Jianzhisu disinfectant, BIOCID-30 and 75% alcohol solution had good killing effect after 1. 5 min respectively with four kinds of bacteria. The 600 times diluted Didecyl dimethyl ammonium bromide solution could effectively kill Staphylococcus aureus,  Klebsiella pneumoniae and Salmonella typhimurium in 5 - 10 min, while its bactericidal effect on Pseudomonas aeruginosa was not ideal. The 200 times diluted Didecyl dimethyl ammonium bromide solution could kill Pseudomonas aeruginosa effectively for 1. 5 min. Conclusion The bromogeramine solution, Jianzhisu disinfectant, BIOCID-30 and 75% alcohol solution could produce good bactericidal effect on four kinds of bacteria within a short time. A higher concentration of Didecyl dimethyl ammonium bromide solution could effectively kill Pseudomonas aeruginosa.

Key words: disinfectants, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, bactericidal effect

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